| 质粒名称: | Flag Depdc5 pRK5 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 46340 |
| 存储实验室: | David Sabatini |
|
载体骨架基本信息 |
|
| 载体名称: | pRK5 |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | 9464 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄青霉素，100μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | -- |
|
插入基因信息 |
|
| 插入基因名称: | Depdc5 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 4746 bp |
| 基因突变: | 与Depdc5同种型1的区别在于从724位开始的10个氨基酸：ILTLSAPPVV |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | 标志（主干上的N终端） |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | SaI (未破坏) |
| 3’克隆位点: | NotI (未破坏) |
| 5’测序引物 | SP6 |
| 3’测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | Flag Depdc5 pRK5 was a gift from David Sabatini (Addgene plasmid # 46340 ; http://n2t.net/addgene:46340 ; RRID:Addgene_46340) |
| 参考文献部分: | A Tumor suppressor complex with GAP activity for the Rag GTPases that signal amino acid sufficiency to mTORC1. Bar-Peled L, Chantranupong L, Cherniack AD, Chen WW, Ottina KA, Grabiner BC, Spear ED, Carter SL, Meyerson M, Sabatini DM. Science. 2013 May 31;340(6136):1100-6. doi: 10.1126/science.1232044. 10.1126/science.1232044 PubMed 23723238 |
买家导航
Flag Depdc5 pRK5载体质粒基本信息
Flag Depdc5 pRK5质粒图谱载体图谱和Flag Depdc5 pRK5载体序列质粒序列多克隆位点信息
Flag Depdc5 pRK5质粒载体简介
Flag Depdc5 pRK5质粒序列载体序列
LOCUS Flag Depdc5 pRK5 9464 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Flag Depdc5 pRK5
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9464)
AUTHORS Bar-Peled L, Chantranupong L, Cherniack AD, Chen WW, Ottina KA,
Grabiner BC, Spear ED, Carter SL, Meyerson M, Sabatini DM
TITLE A Tumor suppressor complex with GAP activity for the Rag GTPases
that signal amino acid sufficiency to mTORC1.
JOURNAL Science. 2013 May 31;340(6136):1100-6. doi: 10.1126/science.1232044.
PUBMED 23723238
REFERENCE 2 (bases 1 to 9464)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9464
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 107..128
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 143..173
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 181..197
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 186..208
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 205..221
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 205..221
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
enhancer 250..629
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 630..833
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 784..804
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 830..854
/label=LNCX
/note="Human CMV promoter, forward primer"
promoter 1064..1082
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind 1064..1081
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind 1095..1114
/label=pMT2-F
/note="Synthetic intron, forward primer"
CDS 1173..1196
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
regulatory 6000..6009
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
polyA_signal 6013..6147
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6050..6069)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 6104..6123
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
promoter 6216..6573
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(6239..6259)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 6424..6559
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6486..6505
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(6593..6610)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(6593..6609)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6602..6624)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 6822..7277
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6909..6928)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 7119..7140
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(7294..7313)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 7413..7435
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(7473..7491)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 7559..7663
/gene="bla"
/label=AmpR promoter
CDS 7664..8524
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7882..7901)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 8695..9283
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 9184..9203
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 9437..9454
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
181 ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg aattaattcg
241 agctcgcccg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
301 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
361 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
421 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
481 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
541 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
601 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
661 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
721 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
781 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag
841 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc
901 gggaccgatc cagcctccgc ggccgggaac ggtgcattgg aacgcggatt ccccgtgcca
961 agagtgacgt aagtaccgcc tatagagtct ataggcccac ccccttggct tcgttagaac
1021 gcggctacaa ttaatacata accttatgta tcatacacat acgatttagg tgacactata
1081 gaataacatc cactttgcct ttctctccac aggtgtccac tcccaggtcc aactgcacct
1141 cggttctatc gattgaattc accatggaaa tggactacaa ggatgacgat gacaagggtg
1201 gtggtggtgg tgcgtcgacg atgagaacaa caaaggtcta caaactcgtc atccacaaga
1261 agggctttgg gggcagtgat gatgagctag ttgtgaaccc caaagtgttc cctcacatca
1321 agcttggaga cattgtagag attgcacacc ccaacgatga atacagccct ctgcttttgc
1381 aggtcaagtc tcttaaggaa gatttacaga aggaaactat cagtgtggac cagactgtga
1441 ctcaagtgtt ccggctgaga ccttatcagg atgtctatgt taatgtcgta gaccctaagg
1501 atgtgaccct tgacctagtg gaattaactt ttaaggatca gtatattggc cgtggggata
1561 tgtggcgact aaagaaaagt ttggtcagca catgtgccta tatcacccag aaggtggagt
1621 ttgctggcat cagagcacag gctggtgaac tgtgggttaa gaatgagaag gtcatgtgtg
1681 gctacatcag tgaagatacc agggtggtgt ttcgttctac gtcggctatg gtttacatat
1741 ttattcagat gagctgtgaa atgtgggatt ttgatattta tggggatttg tattttgaga
1801 aagctgtgaa tggtttcctt gctgatctat ttaccaagtg gaaggagaag aactgtagtc
1861 atgaagtgac agtggtcctg ttttctagaa ctttctatga tgcaaaatct gttgatgaat
1921 ttcctgaaat aaaccgagcc tcaattcgac aggatcacaa ggggagattc tatgaagact
1981 tttacaaagt ggtggtgcag aatgagagaa gagaagaatg gacttcactt ctcgtaacca
2041 ttaaaaaact cttcatccag tatccagtgt tggtgcgact ggaacaggca gagggctttc
2101 ctcaaggaga taattctacc tcagcacaag gaaactacct ggaggccatc aatctgtcat
2161 tcaatgtgtt tgataagcac tacatcaacc gcaactttga ccgaactggg cagatgtcag
2221 tggtgatcac gcccggggtg ggtgtctttg aagtggaccg cctactcatg atcctgacca
2281 agcagcggat gatagataat ggaattggtg tggatttggt gtgcatggga gagcaaccgt
2341 tacatgctgt cccattgttc aagctccata atcggagtgc tccccgtgat tctcgtctgg
2401 gcgatgacta taatatccct cactggataa accacagttt ctacacatcc aaaagccagc
2461 tcttttgtaa tagtttcacc ccacgaataa aactggcagg aaagaagccc gcctctgaga
2521 aagcaaaaaa tggccgtgat acatctctcg ggagtccaaa agaatctgag aacgcccttc
2581 ccatccaagt agattatgac gcctatgacg ctcaagtgtt caggctgccc ggcccatccc
2641 gggcccagtg cctcaccacc tgcagatctg tgcgagagcg agagagtcac agtcgaaaga
2701 gtgccagctc ctgtgatgtt tcatccagcc cttccctacc aagccgcaca ctgcccactg
2761 aggaagtgag gagccaggct tctgacgaca gctccctagg caagagtgcc aacatcctga
2821 tgatcccaca cccccacctg caccagtatg aagtcagcag ctccttggga tacaccagca
2881 ctcgagatgt cctggagaac atgatggagc caccacagcg agactccagt gcaccaggga
2941 ggtttcacgt tggcagtgca gaatccatgc tgcatgttcg acctggtgga tacacgcccc
3001 agagagcact gattaacccc ttcgctccct ctcggatgcc catgaagctt acgtccaaca
3061 gaaggcgctg gatgcacact tttcctgtgg ggccatccgg agaagccatc cagatccacc
3121 accagacccg acagaatatg gcggagctac aaggcagcgg gcagagggat ccaactcact
3181 cctctgcaga gctgctggag ttagcatatc atgaagctgc tggaaggcac agcaattccc
3241 gccagcctgg tgacggcatg tccttcttga acttcagtgg aacagaggag ctttctgtcg
3301 gcctgcttag caacagtggt gcaggtatga atcctaggac ccagaataag gattctctag
3361 aggacagtgt ttctacctct ccagacccaa ttctgacact gtctgctccc cctgtagtgc
3421 caggcttctg ttgcacagtt ggagtggact ggaagtctct cactactccg gcgtgcctcc
3481 cccttaccac cgactacttc cctgaccgcc agggcctgca gaatgactac acagagggct
3541 gttatgatct ccttccagaa gcagacatcg acaggaggga cgaagatggt gtgcagatga
3601 cagcccagca ggtatttgaa gagtttattt gccaacgtct catgcagggc taccaaatca
3661 tagtgcagcc caagacacag aaacccaatc ctgctgtccc gcccccgctg agcagtagcc
3721 cactctatag ccgaggcctt gtgtcccgaa accgccctga ggaggaggac cagtattggc
3781 tgagtatggg cagaacgttc cacaaagtga cgctgaagga taagatgatc acagtgacgc
3841 gataccttcc caagtatcct tatgaatctg cccagatcca ctacacctac agcctctgtc
3901 cttcccactc agactcagag ttcgtctcct gctgggtgga attctcccac gaacggctgg
3961 aggagtacaa gtggaattac ttagatcagt atatctgttc tgccggctct gaagacttca
4021 gcttaattga gtccctgaag ttctggagga cccgcttcct gctgctgcca gcctgtgtca
4081 ccgccaccaa gcgcatcacg gagggggagg cccactgcga catctatggg gacaggcccc
4141 gtgcagacga ggacgagtgg caactcctgg atggttttgt ccgctttgtg gagggcttga
4201 atcgcattcg caggcggcat cgctcggatc gcatgatgcg gaaagggacc gccatgaaag
4261 gcttgcagat gactgggccc atttccacgc attctctgga gtcaactgca cccccagtgg
4321 ggaagaaggg aacctcagct ctctctgccc tgttggagat ggaggccagt cagaagtgcc
4381 tgggagaaca gcaggcagct gtgcatggtg ggaagagctc cgcccagtca gccgagagca
4441 gcagcgttgc catgactccc acctacatgg acagcccacg aaaggtatct gtggaccaaa
4501 cagccactcc tatgttggac ggcaccagtt tgggcatatg cacaggccaa tccatggaca
4561 gaggcaacag ccagaccttt gggaactccc agaacatagg agaacagggc tactcctcca
4621 caaactccag tgacagcagc tctcagcagc tggtggcaag ctccttgacc tcatcctcta
4681 ccctgacaga gatcctggaa gccatgaagc acccctcgac aggagtccag ctgctctctg
4741 aacagaaggg cctctcaccg tactgcttca tcagcgcgga ggtggtacac tggttggtga
4801 accacgtgga ggggatccag acacaggcga tggccattga catcatgcag aaaatgctgg
4861 aagagcagct catcacacat gcatctggcg aagcctggcg gaccttcatc tacggcttct
4921 atttctacaa gatagtaacg gacaaagagc ccgaccgagt ggccatgcag cagcccgcca
4981 ccacctggca cacagcagga gtggacgact tcgccagctt ccagcgcaag tggtttgagg
5041 tggcctttgt ggcagaagag ctcgtgcact ctgagattcc tgcctttctc ctgccctggc
5101 tgcctagccg gccagcctcc tatgcaagta ggcacagctc ctttagccga agttttggag
5161 gacggagcca ggcggcagca cttttagctg ccactgtccc agagcagagg actgtgaccc
5221 tggatgttga cgtgaacaac cgcacagacc ggctggagtg gtgcagctgt tattaccatg
5281 gcaacttttc tctgaatgca gcctttgaga tcaagctgca ctggatggcg gtgaccgcag
5341 cagtactctt cgagatggtc caaggttggc atcggaaagc cacctcctgt ggcttcttgt
5401 tagtcccagt tttggagggg ccttttgcac tgcccagtta cctgtatggc gacccccttc
5461 gtgcccagct cttcatccca ctcaacatca gctgcttgct caaggagggc agcgagcacc
5521 tgtttgatag ctttgaaccc gaaacgtact gggatcgaat gcacctcttc caggaagcca
5581 ttgcacacag gtttgggttt gtacaagata aatattctgc ctctgctttt aacttccctg
5641 ctgagaacaa gcctcagtat atccacgtta caggaacagt gtttctgcag ctgccctact
5701 ccaagcgcaa gttctcaggg cagcagcggc ggcggcggaa ctccaccagc tccaccaacc
5761 agaacatgtt ctgcgaggag cgggtcggct acaactgggc ctacaacacc atgctcacca
5821 aaacatggcg ctccagcgcc acaggggatg aaaagtttgc tgatcggctg ctgaaggact
5881 tcacggactt ctgcatcaac cgtgacaacc ggctggtcac gttctggaca agttgcctgg
5941 agaagatgca tgccagtgcc ccgtgagcgg ccgctaagta agtaaggatc cccagcttgg
6001 ccgccatggc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
6061 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
6121 atcaatgtat cttatcatgt ctggatcggg aattaattcg gcgcagcacc atggcctgaa
6181 ataacctctg aaagaggaac ttggttaggt accttctgag gcggaaagaa ccagctgtgg
6241 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
6301 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
6361 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
6421 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
6481 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
6541 ggaggctttt ttggaggcct aggcttttgc aaaaagctgt taacagcttg gcactggccg
6601 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
6661 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
6721 aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc
6781 tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc
6841 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
6901 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
6961 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
7021 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
7081 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
7141 aacaacactc aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc
7201 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
7261 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
7321 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
7381 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
7441 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
7501 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
7561 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
7621 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
7681 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
7741 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
7801 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
7861 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
7921 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
7981 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
8041 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
8101 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
8161 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
8221 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
8281 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
8341 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
8401 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
8461 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
8521 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
8581 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
8641 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
8701 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
8761 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
8821 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
8881 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
8941 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
9001 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
9061 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
9121 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
9181 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
9241 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
9301 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
9361 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
9421 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaag
//
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